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carborundum virus inoculation

Experimental Inoculation of Plants and Animals with …

21/12/2010· In addition, virus was recovered from the feces of a fruit bat on day 21 postinoculation. Virus was also recovered on a few occasions from snakes, and NIH mice and spiders (), but this was at a minimal titer of 10 1.0 FFU/ml and could represent residual infectivity

Paenibacillus lentimorbus Inoculation Enhances Tobacco …

2/3/2016· Soil inoculation of B-30488 enhanced the plant vigor while significantly decreased the virulence and virus RNA accumulation by ~12 fold (91%) in systemic leaves of CMV infected tobacco plants as compared to the control ones.

Ultra-sensitive detection of papaya ringspot virus using single …

with carborundum. Leaves with inoculum were gently rubbed with glass rods and then rinsed in dH 2 O. Inoculated plants were kept in the greenhouse for symptom development. Young leaves were collected from the inoculated plants at 7-day intervals, dpi.

Experimental Inoculation of Plants and Animals with Ebola Virus

virus mixed with Carborundum powder was rubbed gently with cotton buds onto two leaves on each of the plant varieties on experiment; to simulate vector-borne trans-mission, a second plant of each type was inoculated with 1.0µl virus suspension into Table 1.

Potyvirus virion structure shows conserved protein fold …

1/9/2017· WMV inoculation and purifiion Carborundum-dusted cotyledons of squash plants were inoculated with an homogenate consisting of a dried material from …

Routes of Viral Inoculation in Eryonated Eggs …

Draw a line on the shell marking the air space. With a sterile egg shell puncher or carborundum disc pierce a hole in the end of the egg at the marked inoculation site. Attach a needle to 1 ml syringe. Draw 0.1ml of inoculums (Viral suspension) into 1 ml syringe.

Ultra-sensitive detection of papaya ringspot virus using single …

with carborundum. Leaves with inoculum were gently rubbed with glass rods and then rinsed in dH 2 O. Inoculated plants were kept in the greenhouse for symptom development. Young leaves were collected from the inoculated plants at 7-day intervals, dpi.

Responses of white yam (Dioscorea rotundata) cultivars …

28/4/2004· For inoculation of DaBV, the resuspended virus was diluted in inoculation buffer and inoculated onto carborundum‐dusted leaves, as described above. Vector transmission of yam viruses Ten plants (five plants in two replies placed in two different cages) per cultivar at the third leaf stage were selected for transmission experiments for each virus.

Factors Affecting Mechanical Transmission of Tomato …

The most susceptible growth stage of peanut to TSWV inoculation was 2 to 3 days after germination (6 to 7 days after planting). The inoculation protocol consistently resulted in a higher percentage of infected plants from different sources of inoculum such as infected peanut, tobacco, and tomato.

Bean Common Mosaic Virus -BCMV Bean Common Mosaic Necrosis Virus -BCMNV

inoculation with the virus. The I gene gives immunity to BCMV isolates at temperatures less than 30C. For some BCMV isolates at temperatures greater than 30C and for all BCMNV susceptible cultivars infected with BCMNV and held at any growing

OF TOBACCO MOSAIC VIRUS (l)

inoculation method got best results with carborundum 1000 mesh. Beraha et al. (1955) found a relationship between the particle size of the abrasive (aluminium oxide or silicon carbide) and the amount of infection. The smaller the particles the greater thç

NATIONAL PLANT DIAGNOSTIC NETWORK VIRUS DIAGNOSTICS …

• ABRASIVES ARE TYPICALLY USED TO MAKE MINOR WOUNDS TO ALLOW VIRUS ENTRY - Carborundum or corundum dusted on leaves to be inoculated, or Celite mixed with the inoculum. MECHANICAL INOCULATION Select appropriate source

Impact of Cowpea mottle virus on the Growth and Yield …

The virus was first propagated by mechanical inoculation of cowpea plants kept in insect-proof cages. Two weeks after inoculation, symptom-bearing leaves from infected plants were harvested. Leaf samples were ground at the ratio of 1/10 (w/v) in 0.01 M sodium phosphate buffer, pH 7.4.

Experimental Inoculation of Plants and Animals with Ebola Virus

virus mixed with Carborundum powder was rubbed gently with cotton buds onto two leaves on each of the plant varieties on experiment; to simulate vector-borne trans-mission, a second plant of each type was inoculated with 1.0µl virus suspension into Table 1.

PROTECTION BETWEEN STRAINS OF Papaya ringspot virus - TYPE …

inoculation, a systemic superinfection occurred in some plants. All plants became protected against the expression of symptoms induced by the severe strain when the challenge inoculation was made

Mechanical inoculation of plant viruses

This technique is for the mechanical inoculation of viruses to plants. It is used to diagnose a virus by its reactions in a variety of plant species, to test the infectivity of virus samples using local lesion hosts, and to propagate viruses. The virus preparation is rubbed onto the surface of the l …. This technique is for the mechanical

Ultra-sensitive detection of papaya ringspot virus using single …

with carborundum. Leaves with inoculum were gently rubbed with glass rods and then rinsed in dH 2 O. Inoculated plants were kept in the greenhouse for symptom development. Young leaves were collected from the inoculated plants at 7-day intervals, dpi.

Different Plant Viruses Induce Changes in Feeding …

31/1/2020· Two lower leaves were dusted with Carborundum (SiC), which was used to abrade the leaf during mechanical inoculation to aid virus entry. The sap was rubbed gently onto the two leaves and then the excess sap and ground leaf debris cleaned off by spraying the leaf with distilled water.

Effect of time of inoculation and light conditions on the …

ment of local lesions on C. pendulum following PVX inoculation, is influenced by the time of inoculation during the day as well as by the post-inoculation light conditions. It is also indied that the virus can multiply in the absence of light

(PDF) DNA abrasion onto plants is an effective method …

A method was analyzed for inoculating geminiviruses using plasmid DNA rubbed onto leaves in the presence of an abrasive (DNA abrasion). Although the use of DNA abrasion to inoculate geminiviruses has been described previously, the technique has fallen out …

Evaluation of disinfectants to prevent mechanical …

27/1/2015· It took approximately 5 weeks post inoculation for PepMV to be detectable by ELISA achieving a 100% infection rate in plants from the positive control. In comparison, it typically took approximately only 1–3 weeks for PepMV to be detectable by ELISA in rub-inoculated plants.

Dalmatian Toadflax (Linaria dalmatica): New Host for Cucuer Mosaic Virus

with carborundum, the inoculation buffer was spread along 5 to 10 leaves per plant using a Q-tip for the 18 CMV-inoculated treatment plants. Four weeks after inoculation, plants were tested for the presence of CMV using an enzyme-linked immunosorbent 6 2

Virus Diseases of Cereal Crops in South Dakota

Method of Inoculation dum-ruh method mixing a grade carborundum powder ( grain) the diluted sap from eased leaves. This inoculum was then applied to the leaves of the plants according to the technique developed by McKinney ( 8). Although a proportion

Paenibacillus lentimorbus Inoculation Enhances …

2/3/2016· B-30488 inoculation has favorably maneuvered the essential biophysical (ion leakage and photosynthetic efficiency) and biochemical (sugar, proline, chlorophyll, malondialdehyde, acid phosphatase and alkaline phosphatase) attributes of tobacco plants to …

Weeds experimentally infected with beet-mosaic virus …

Weeds grown from seeds were experimentally infected with the virus by mechanical inoculation using the carborundum method described by (Rawlins and Tompkins (1936)). The virus was recovered from each species of weed and transferred to sugar

Some Characteristics of the Wheat Streak-Mosaic Virus and Disease

the carborundum leaf-rubbing method described by McKinney (6) and used also by Slykhuis (9) to be the most successful and consistent method of inoculation for small populations. For larger populations a high pressure spraying system originally described by

OF TOBACCO MOSAIC VIRUS (l)

inoculation method got best results with carborundum 1000 mesh. Beraha et al. (1955) found a relationship between the particle size of the abrasive (aluminium oxide or silicon carbide) and the amount of infection. The smaller the particles the greater thç

Paenibacillus lentimorbus Inoculation Enhances …

2/3/2016· B-30488 inoculation has favorably maneuvered the essential biophysical (ion leakage and photosynthetic efficiency) and biochemical (sugar, proline, chlorophyll, malondialdehyde, acid phosphatase and alkaline phosphatase) attributes of tobacco plants to …

Some Characteristics of the Wheat Streak-Mosaic Virus and Disease

the carborundum leaf-rubbing method described by McKinney (6) and used also by Slykhuis (9) to be the most successful and consistent method of inoculation for small populations. For larger populations a high pressure spraying system originally described by

Interactions of viruses in Cowpea: effects on growth …

8/2/2007· Generally, the growth and yield of the buffer inoculated control plants were significantly higher than those of the virus inoculated plants. Inoculation of plants at an early age of 10 DAP resulted in more severe effect than inoculations at a later stage of 30 DAP.